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i scei expression vector pcbasce  (Addgene inc)


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    Structured Review

    Addgene inc i scei expression vector pcbasce
    I Scei Expression Vector Pcbasce, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/i+scei+expression+vector+pcbasce/pCBASceI+(Plasmid+%2326477)/pmc12256224-65-13-17
    Average 96 stars, based on 293 article reviews
    i scei expression vector pcbasce - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: PLK1 Phosphorylates WRN at Replication Forks
    Article Snippet: Prostate cancer, particularly castration-resistant prostate cancer, remains a serious public health issue.. Androgen signaling inhibitors have emerged as a major treatment approach but with limited success.. Thus, identification of novel treatment targets is of high clinical relevance.

    Article Title: PALB2 deficiency may sensitize H3K27M-mutant pediatric HGG cells to BMN673/talazoparib
    Article Snippet: .. The indicated EJ5 reporter cell lines were transfected with two plasmids, namely, the I-SceI expression vector pCBASce (Addgene #26477) and the DsRed expression vector (Addgene #54493), 48 h later, the percentage of GFP-positive cells among DsRed-positive cells was evaluated by imaging with a Carl Zeiss LSM 710 confocal fluorescence microscope and quantified via FACS on a BD AccuriTM C6 flow cytometer (BD Biosciences, USA) using BD AccuriTM software. .. Subcellular protein fractions were collected with a subcellular fractionation kit (Thermo Fisher Scientific, USA).

    Plasmid Preparation:

    Article Title: PLK1 Phosphorylates WRN at Replication Forks
    Article Snippet: Prostate cancer, particularly castration-resistant prostate cancer, remains a serious public health issue.. Androgen signaling inhibitors have emerged as a major treatment approach but with limited success.. Thus, identification of novel treatment targets is of high clinical relevance.

    Article Title: Different SWI/SNF complexes coordinately promote R-loop- and RAD52-dependent transcription-coupled homologous recombination
    Article Snippet: .. Cells were treated with siRNAs and subsequently transfected with I-SceI-expression vector pCBASce (a gift from Maria Jasin; Addgene plasmid #26477) ( ). ..

    Transfection:

    Article Title: PLK1 Phosphorylates WRN at Replication Forks
    Article Snippet: Prostate cancer, particularly castration-resistant prostate cancer, remains a serious public health issue.. Androgen signaling inhibitors have emerged as a major treatment approach but with limited success.. Thus, identification of novel treatment targets is of high clinical relevance.

    Article Title: Different SWI/SNF complexes coordinately promote R-loop- and RAD52-dependent transcription-coupled homologous recombination
    Article Snippet: .. Cells were treated with siRNAs and subsequently transfected with I-SceI-expression vector pCBASce (a gift from Maria Jasin; Addgene plasmid #26477) ( ). ..

    Article Title: PALB2 deficiency may sensitize H3K27M-mutant pediatric HGG cells to BMN673/talazoparib
    Article Snippet: .. The indicated EJ5 reporter cell lines were transfected with two plasmids, namely, the I-SceI expression vector pCBASce (Addgene #26477) and the DsRed expression vector (Addgene #54493), 48 h later, the percentage of GFP-positive cells among DsRed-positive cells was evaluated by imaging with a Carl Zeiss LSM 710 confocal fluorescence microscope and quantified via FACS on a BD AccuriTM C6 flow cytometer (BD Biosciences, USA) using BD AccuriTM software. .. Subcellular protein fractions were collected with a subcellular fractionation kit (Thermo Fisher Scientific, USA).

    Imaging:

    Article Title: PALB2 deficiency may sensitize H3K27M-mutant pediatric HGG cells to BMN673/talazoparib
    Article Snippet: .. The indicated EJ5 reporter cell lines were transfected with two plasmids, namely, the I-SceI expression vector pCBASce (Addgene #26477) and the DsRed expression vector (Addgene #54493), 48 h later, the percentage of GFP-positive cells among DsRed-positive cells was evaluated by imaging with a Carl Zeiss LSM 710 confocal fluorescence microscope and quantified via FACS on a BD AccuriTM C6 flow cytometer (BD Biosciences, USA) using BD AccuriTM software. .. Subcellular protein fractions were collected with a subcellular fractionation kit (Thermo Fisher Scientific, USA).

    Fluorescence:

    Article Title: PALB2 deficiency may sensitize H3K27M-mutant pediatric HGG cells to BMN673/talazoparib
    Article Snippet: .. The indicated EJ5 reporter cell lines were transfected with two plasmids, namely, the I-SceI expression vector pCBASce (Addgene #26477) and the DsRed expression vector (Addgene #54493), 48 h later, the percentage of GFP-positive cells among DsRed-positive cells was evaluated by imaging with a Carl Zeiss LSM 710 confocal fluorescence microscope and quantified via FACS on a BD AccuriTM C6 flow cytometer (BD Biosciences, USA) using BD AccuriTM software. .. Subcellular protein fractions were collected with a subcellular fractionation kit (Thermo Fisher Scientific, USA).

    Microscopy:

    Article Title: PALB2 deficiency may sensitize H3K27M-mutant pediatric HGG cells to BMN673/talazoparib
    Article Snippet: .. The indicated EJ5 reporter cell lines were transfected with two plasmids, namely, the I-SceI expression vector pCBASce (Addgene #26477) and the DsRed expression vector (Addgene #54493), 48 h later, the percentage of GFP-positive cells among DsRed-positive cells was evaluated by imaging with a Carl Zeiss LSM 710 confocal fluorescence microscope and quantified via FACS on a BD AccuriTM C6 flow cytometer (BD Biosciences, USA) using BD AccuriTM software. .. Subcellular protein fractions were collected with a subcellular fractionation kit (Thermo Fisher Scientific, USA).

    FACS:

    Article Title: PALB2 deficiency may sensitize H3K27M-mutant pediatric HGG cells to BMN673/talazoparib
    Article Snippet: .. The indicated EJ5 reporter cell lines were transfected with two plasmids, namely, the I-SceI expression vector pCBASce (Addgene #26477) and the DsRed expression vector (Addgene #54493), 48 h later, the percentage of GFP-positive cells among DsRed-positive cells was evaluated by imaging with a Carl Zeiss LSM 710 confocal fluorescence microscope and quantified via FACS on a BD AccuriTM C6 flow cytometer (BD Biosciences, USA) using BD AccuriTM software. .. Subcellular protein fractions were collected with a subcellular fractionation kit (Thermo Fisher Scientific, USA).

    Flow Cytometry:

    Article Title: PALB2 deficiency may sensitize H3K27M-mutant pediatric HGG cells to BMN673/talazoparib
    Article Snippet: .. The indicated EJ5 reporter cell lines were transfected with two plasmids, namely, the I-SceI expression vector pCBASce (Addgene #26477) and the DsRed expression vector (Addgene #54493), 48 h later, the percentage of GFP-positive cells among DsRed-positive cells was evaluated by imaging with a Carl Zeiss LSM 710 confocal fluorescence microscope and quantified via FACS on a BD AccuriTM C6 flow cytometer (BD Biosciences, USA) using BD AccuriTM software. .. Subcellular protein fractions were collected with a subcellular fractionation kit (Thermo Fisher Scientific, USA).

    Software:

    Article Title: PALB2 deficiency may sensitize H3K27M-mutant pediatric HGG cells to BMN673/talazoparib
    Article Snippet: .. The indicated EJ5 reporter cell lines were transfected with two plasmids, namely, the I-SceI expression vector pCBASce (Addgene #26477) and the DsRed expression vector (Addgene #54493), 48 h later, the percentage of GFP-positive cells among DsRed-positive cells was evaluated by imaging with a Carl Zeiss LSM 710 confocal fluorescence microscope and quantified via FACS on a BD AccuriTM C6 flow cytometer (BD Biosciences, USA) using BD AccuriTM software. .. Subcellular protein fractions were collected with a subcellular fractionation kit (Thermo Fisher Scientific, USA).



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